Differentiation of Mouse Embryonic Stem Cell into Insulin-Secreting Cell

Purpose: Differentiation of mouse embryonic stem cells into Insulin secreting endocrine cells. Materials and Methods: In this study, Royan B1 mouse embryonic stem cell (derived from C57BL/6 mouse) were used. In directed differentiation method, embryonicstem cells after embryoid bodies formation were differentiated into insulin secreting cells. Nestin positive cells were obtained after culture in selection medium. In final stage, these cells were expanded in the presence of bFGF, followed by addition of Nicotinamid to promote differentiation of insulin- secreting cells.Cells were assayed by immunocytochemistry, RT-PCR and insulin secreting assay with Radio-Immuno assay kit. Results: Analysis of differentiated cells immunocytochemistry showed that these cells can be insulin, Glucagon, somatostatin and polypeptide pancreatic. RT-PCR reaction demonstrated the expression of insulin and Glucogon genes. Differentiated cells secreted insulin in response to Glucose, but no significance difference in insulin concentration was observed when concentration of glucose was increased. Conclusion: Results of this study showed that mouse embryonic stem cells can differentiated into insulin-secreting cells. But it seems that these cells aren't real beta-cell.